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Fanconi Anemia ( FA ) is a rare autosomal recessive disorder characterized by hypersensitivity to inter-strand crosslinks ( ICLs ) . FANCD2 , a central factor of the FA pathway , is essential for the repair of double strand breaks ( DSBs ) generated during fork collapse at ICLs . While lesions different from ICLs can a...
Here we show that irradiation with low doses of UV light causes modest accumulation of replication-coupled double strand breaks ( DSBs ) , i . e . collapsed forks . Remarkably , the Fanconi Anemia protein FANCD2 is central to prevent the aberrant processing of UV-triggered DSBs and the generation of micronuclei and chr...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2016
Chromosomal Integrity after UV Irradiation Requires FANCD2-Mediated Repair of Double Strand Breaks
The use of the bacterium Wolbachia is an attractive alternative method to control vector populations . In mosquitoes , as in members of the Culex pipiens complex , Wolbachia induces a form of embryonic lethality called cytoplasmic incompatibility , a sperm-egg incompatibility occurring when infected males mate either w...
Mosquitoes of the Culex pipiens complex are important vectors of human pathogens including filarial parasites and many currently expanding arboviruses . The absence of effective vaccines and the evolution of insecticide resistance stress the urgent need for the development of novel control strategies . One strategy tha...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "vector", "biology", "biology", "microbiology" ]
2011
Cytoplasmic Incompatibility as a Means of Controlling Culex pipiens quinquefasciatus Mosquito in the Islands of the South-Western Indian Ocean
Rabies is a fatal zoonosis that still causes nearly 70 , 000 human deaths every year . In Europe , the oral rabies vaccination ( ORV ) of red foxes ( Vulpes vulpes ) was developed in the late 1970s and has demonstrated its effectiveness in the eradication of the disease in Western and some Central European countries . ...
This paper reviews ten years of rabies epidemiology in the three Baltic countries . Both surveillance efforts and oral rabies vaccination campaigns have resulted in the near eradication of the disease . Multivariate analysis assessed with generalized linear models ( GLM ) suggested lower oral vaccination effectiveness ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "tropical", "diseases", "microbiology", "vertebrates", "geographical", "locations", "estonia", "dogs", "animals", "mammals", "viruses", "vaccines", "preventiv...
2016
Rabies in the Baltic States: Decoding a Process of Control and Elimination
Spatial modeling is increasingly utilized to elucidate relationships between demographic , environmental , and socioeconomic factors , and infectious disease prevalence data . However , there is a paucity of studies focusing on spatio-temporal modeling that take into account the uncertainty of diagnostic techniques . W...
Schistosomiasis is a serious public health problem in the People's Republic of China and elsewhere , and mapping of risk areas is important for guiding control interventions . Here , a 10-year surveillance database from Dangtu County in the southeastern part of the People's Republic of China was utilized for modeling t...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "public", "health", "and", "epidemiology/epidemiology", "infectious", "diseases/helminth", "infections", "public", "health", "and", "epidemiology/social", "and", "behavioral", "determinants", "of", "health", "mathema...
2008
Bayesian Spatio-Temporal Modeling of Schistosoma japonicum Prevalence Data in the Absence of a Diagnostic ‘Gold’ Standard
Staphylococcus aureus is a human commensal that can also cause systemic infections . This transition requires evasion of the immune response and the ability to exploit different niches within the host . However , the disease mechanisms and the dominant immune mediators against infection are poorly understood . Previous...
Staphylococcus aureus is a major human pathogen that causes a wide variety of infections . In animal infection models , high doses of S . aureus are generally required to establish infections . We have recently shown in animal models that this is due to very few bacteria within the infecting population going on to caus...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "&", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "fish", "liver", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "vertebrates", "staphylococcus", "aureus", "animals", "animal", "models", "osteichthye...
2018
Staphylococcus aureus infection dynamics
Novel drugs are required for the elimination of infections caused by filarial worms , as most commonly used drugs largely target the microfilariae or first stage larvae of these infections . Previous studies , conducted in vitro , have shown that inhibition of Hsp90 kills adult Brugia pahangi . As numerous small molecu...
Adult filarial worms are long-lived nematode parasites that have proved very difficult to kill with existing drugs . Current campaigns for the control or elimination of these parasites are largely based on treatment with drugs such as diethylcarbamazine or ivermectin , that preferentially kill the first stage larvae of...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "oncology", "medicine", "chemotherapy", "and", "drug", "treatment", "cancer", "treatment", "biology", "microbiology", "parasitology" ]
2014
A Repurposing Strategy for Hsp90 Inhibitors Demonstrates Their Potency against Filarial Nematodes
Spikelets are small spike-like depolarizations that can be measured in somatic intracellular recordings . Their origin in pyramidal neurons remains controversial . To explain spikelet generation , we propose a novel single-cell mechanism: somato-dendritic input generates action potentials at the axon initial segment th...
Action potentials ( APs ) are digital , all-or-none signals by which neurons communicate with each other . Therefore , APs are the basis of neural function , yet some of their fundamental features are still not well understood . Here we focus on pyramidal cells , which are the principal neurons in neocortex and hippoca...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "cell", "physiology", "medicine", "and", "health", "sciences", "action", "potentials", "nervous", "system", "depolarization", "membrane", "potential", "junctional", "complexes", "electrophysiology", "neuroscience", "gap", "junctions", "ion", "channels", "nerve", "fibers",...
2017
Spikelets in Pyramidal Neurons: Action Potentials Initiated in the Axon Initial Segment That Do Not Activate the Soma
The bloodstream forms of Trypanosoma brucei , the causative agent of sleeping sickness , rely solely on glycolysis for ATP production . It is generally accepted that pyruvate is the major end-product excreted from glucose metabolism by the proliferative long-slender bloodstream forms of the parasite , with virtually no...
Many protists , including parasitic helminthes , trichomonads and trypanosomatids , produce acetate in their mitochondrion or mitochondrion-like organelle , which is excreted as a main metabolic end-product of their energy metabolism . We have recently demonstrated that mitochondrial production of acetate is essential ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2013
Revisiting the Central Metabolism of the Bloodstream Forms of Trypanosoma brucei: Production of Acetate in the Mitochondrion Is Essential for Parasite Viability
DNA molecules are highly charged semi-flexible polymers that are involved in a wide variety of dynamical processes such as transcription and replication . Characterizing the binding landscapes around DNA molecules is essential to understanding the energetics and kinetics of various biological processes . We present a c...
Protein-DNA and ion-DNA interactions are key for many essential biological activities such as DNA condensation , replication , transcription and repair . All these processes require DNA-binding proteins to associate tightly to their specific targets , while also being able to find these sites in an efficient way . To f...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
DNA Exit Ramps Are Revealed in the Binding Landscapes Obtained from Simulations in Helical Coordinates
Theileria parasites invade and transform bovine leukocytes causing either East Coast fever ( T . parva ) , or tropical theileriosis ( T . annulata ) . Susceptible animals usually die within weeks of infection , but indigenous infected cattle show markedly reduced pathology , suggesting that host genetic factors may cau...
Theileria annulata causes tropical theileriosis that is endemic in cattle in North Africa , the Middle East , India and China . T . parva causes East Coast fever that is prevalent in East and Southern Africa . In endemic countries indigenous cattle are more resistant to pathology , but produce little meat and milk and ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/protozoal", "infections", "cell", "biology/extra-cellular", "matrix", "cell", "biology/leukocyte", "signaling", "and", "gene", "expression" ]
2010
TGF-b2 Induction Regulates Invasiveness of Theileria-Transformed Leukocytes and Disease Susceptibility
No-go Decay ( NGD ) is a process that has evolved to deal with stalled ribosomes resulting from structural blocks or aberrant mRNAs . The process is distinguished by an endonucleolytic cleavage prior to degradation of the transcript . While many of the details of the pathway have been described , the identity of the en...
In all organisms , optimum cellular fitness depends on the ability of cells to recognize and degrade aberrant molecules . Messenger RNA is subject to alterations and , as a result , often presents roadblocks for the translating ribosomes . It is not surprising , then , that organisms evolved pathways to resolve these v...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "northern", "analysis", "deletion", "mutation", "molecular", "probe", "techniques", "messenger", "rna", "polyribosomes", "mutation", "fungi", "molecular", "biology", "techniques", "cellular", "structures", "and", "organelles", "gel", "electrophoresis", "research", "and", ...
2018
Interactions between the mRNA and Rps3/uS3 at the entry tunnel of the ribosomal small subunit are important for no-go decay
Viruses in the Flavivirus genus of the Flaviviridae family are arthropod-transmitted and contribute to staggering numbers of human infections and significant deaths annually across the globe . To identify cellular factors with antiviral activity against flaviviruses , we screened a cDNA library using an iterative appro...
Viruses in the Flavivirus genus are transmitted by arthropods and cause significant disease burden across the globe . We undertook a screening approach to select for and identify host factors that provide resistance to death caused by infection with the mosquito-transmitted Flavivirus , yellow fever virus ( YFV ) . We ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "cell", "biology/membranes", "and", "sorting", "virology/viral", "replication", "and", "gene", "regulation", "virology/mechanisms", "of", "resistance", "and", "susceptibility,", "including", "host", "genetics" ]
2011
Identification and Characterization of the Host Protein DNAJC14 as a Broadly Active Flavivirus Replication Modulator
The population genetic perspective is that the processes shaping genomic variation can be revealed only through simultaneous investigation of sequence polymorphism and divergence within and between closely related species . Here we present a population genetic analysis of Drosophila simulans based on whole-genome shotg...
Population genomics , the study of genome-wide patterns of sequence variation within and between closely related species , can provide a comprehensive view of the relative importance of mutation , recombination , natural selection , and genetic drift in evolution . It can also provide fundamental insights into the biol...
[ "Abstract", "Introduction", "Results/Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "computational", "biology", "evolutionary", "biology", "genetics", "and", "genomics" ]
2007
Population Genomics: Whole-Genome Analysis of Polymorphism and Divergence in Drosophila simulans
In both humans and Drosophila melanogaster , UDP-galactose 4′-epimerase ( GALE ) catalyzes two distinct reactions , interconverting UDP-galactose ( UDP-gal ) and UDP-glucose ( UDP-glc ) in the final step of the Leloir pathway of galactose metabolism , and also interconverting UDP-N-acetylgalactosamine ( UDP-galNAc ) an...
In this manuscript we apply a fruit fly model to explore the relative contributions of each of two different activities attributed to a single enzyme—UDP-galactose 4′-epimerase ( GALE ) ; partial impairment of human GALE results in the potentially severe metabolic disorder epimerase deficiency galactosemia . One GALE a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology", "model", "organisms", "genetics", "biology", "molecular", "cell", "biology", "genetics", "and", "genomics" ]
2012
UDP-Galactose 4′-Epimerase Activities toward UDP-Gal and UDP-GalNAc Play Different Roles in the Development of Drosophila melanogaster
The identification and characterization of antigens expressed in Trypanosoma cruzi stages that parasitize mammals are essential steps for the development of new vaccines and diagnostics . Genes that are preferentially expressed in trypomastigotes may be involved in key processes that define the biology of trypomastigot...
Chagas' disease , caused by the kinetoplastid protozoan parasite Trypanosoma cruzi , is endemic in Latin America . At present there are neither vaccines for prevention nor totally effective drugs for the treatment of the disease . T . cruzi has a complex life cycle alternating between a reduviid insect ( the vector ) a...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "genetics", "and", "genomics/gene", "discovery", "genetics", "and", "genomics/gene", "expression", "infectious", "diseases/neglected", "tropical", "diseases", "microbiology/parasitology", "infectious", "diseases/protozoal", "infections", "genetics", "and", "genomics/bioinformatic...
2010
TcTASV: A Novel Protein Family in Trypanosoma cruzi Identified from a Subtractive Trypomastigote cDNA Library
The interaction of Mycobacterium tuberculosis ( Mtb ) with host cell death signaling pathways is characterized by an initial anti-apoptotic phase followed by a pro-necrotic phase to allow for host cell exit of the bacteria . The bacterial modulators regulating necrosis induction are poorly understood . Here we describe...
Mycobacterium tuberculosis ( Mtb ) , the causative agent of tuberculosis , is a highly successful human pathogen . Following entry into host phagocytic cells , Mtb resides within a modified phagosomal compartment and inhibits apoptotic host cell death . Recent studies have demonstrated that Mtb eventually translocates ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "flow", "cytometry", "cell", "death", "medicine", "and", "health", "sciences", "autophagic", "cell", "death", "pathology", "and", "laboratory", "medicine", "viral", "transmission", "and", "infection", "cell", "processes", "microbiology", "signs", "and", "symptoms", ...
2016
Identification of a Transcription Factor That Regulates Host Cell Exit and Virulence of Mycobacterium tuberculosis
The agr quorum-sensing system of Staphylococcus aureus modulates the expression of virulence factors in response to autoinducing peptides ( AIPs ) . Recent studies have suggested a role for the agr system in S . aureus biofilm development , as agr mutants exhibit a high propensity to form biofilms , and cells dispersin...
A biofilm is a surface-attached community of cells bound together by an extracellular matrix . In a bacterial infection , biofilm-encased cells are protected from antibiotic therapy and host immune response , and these encased cells can develop into a chronic infection . Staphylococcus aureus is a prominent bacterial p...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases/bacterial", "infections", "microbiology/microbial", "growth", "and", "development", "microbiology/cellular", "microbiology", "and", "pathogenesis", "microbiology/medical", "microbiology", "infectious", "diseases/antimicrobials", "and", "drug", "resistance" ]
2008
agr-Mediated Dispersal of Staphylococcus aureus Biofilms
Although malaria has been the leading cause of fever for many years , with improved control regimes malaria transmission , morbidity and mortality have decreased . Recent studies have increasingly demonstrated the importance of non-malaria fevers , which have significantly improved our understanding of etiologies of fe...
Understanding the awareness of the community on non-malaria febrile illnesses is crucial , especially during the recent decline of malaria episodes of malaria . This study conducted focus group discussions with communities to assess their awareness of non-malaria febrile illnesses . In addition , in-depth interviews wi...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "biology", "and", "life", "sciences", "veterinary", "science", "medicine", "and", "health", "sciences", "social", "sciences" ]
2014
Community Knowledge and Attitudes and Health Workers' Practices regarding Non-malaria Febrile Illnesses in Eastern Tanzania
The simultaneous targeting of host and pathogen processes represents an untapped approach for the treatment of intracellular infections . Hypoxia-inducible factor-1 ( HIF-1 ) is a host cell transcription factor that is activated by and required for the growth of the intracellular protozoan parasite Toxoplasma gondii at...
Understanding how a compound blocks growth of an intracellular pathogen is important not only for developing these compounds into drugs that can be prescribed to patients , but also because these data will likely provide novel insight into the biology of these pathogens . Forward genetic screens are one established app...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "signal", "transduction", "medicine", "and", "health", "sciences", "molecular", "cell", "biology", "pathology", "and", "laboratory", "medicine", "cell", "biology", "host-pathogen", "interactions", "genetic", "screens", "medical", "microbiology", "gene", "identification", ...
2014
Forward Genetic Screening Identifies a Small Molecule That Blocks Toxoplasma gondii Growth by Inhibiting Both Host- and Parasite-Encoded Kinases
We introduce a series of experimental procedures enabling sensitive calcium monitoring in T cell populations by confocal video-microscopy . Tracking and post-acquisition analysis was performed using Methods for Automated and Accurate Analysis of Cell Signals ( MAAACS ) , a fully customized program that associates a hig...
The adaptive immune response to pathogen invasion requires the stimulation of lymphocytes by antigen-presenting cells . We hypothesized that investigating the dynamics of the T lymphocyte activation by monitoring intracellular calcium fluctuations might help explain the high specificity and selectivity of this phenomen...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
Barcoding T Cell Calcium Response Diversity with Methods for Automated and Accurate Analysis of Cell Signals (MAAACS)
There is an ultimate need for efficacious vaccines against human cytomegalovirus ( HCMV ) , which causes severe morbidity and mortality among neonates and immunocompromised individuals . In this study we explored synthetic long peptide ( SLP ) vaccination as a platform modality to protect against mouse CMV ( MCMV ) inf...
The majority of infections with the betaherpesvirus human cytomegalovirus ( HCMV ) are clinically unnoticed , but in immunocompromised hosts HCMV infections can be severe and even fatal . Here we investigated in preclinical mouse models the efficacy and mechanisms of synthetic long peptide ( SLP ) -based vaccines elici...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "immunology", "cytomegalovirus", "infection", "vaccines", "preventive", "medicine", "developmental", "biology", "clinical", "...
2016
The Breadth of Synthetic Long Peptide Vaccine-Induced CD8+ T Cell Responses Determines the Efficacy against Mouse Cytomegalovirus Infection
Pervasive natural selection can strongly influence observed patterns of genetic variation , but these effects remain poorly understood when multiple selected variants segregate in nearby regions of the genome . Classical population genetics fails to account for interference between linked mutations , which grows increa...
A central goal of evolutionary genetics is to understand how natural selection influences DNA sequence variability . Yet while empirical studies have uncovered significant evidence for selection in many natural populations , a rigorous characterization of these selection pressures has so far been difficult to achieve ....
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "ecological", "metrics", "mutation", "population", "size", "genetic", "polymorphism", "ecology", "effective", "population", "size", "natural", "selection", "genetics", "biology", "and", "life", "sciences", "population", "genetics", "evolutionary", "biology", "evolutionary...
2014
Genetic Diversity in the Interference Selection Limit
We analyzed the transcriptional signatures of mouse bone marrow-derived macrophages at different times after infection with promastigotes of the protozoan parasite Leishmania major . Ingenuity Pathway Analysis revealed that the macrophage metabolic pathways including carbohydrate and lipid metabolisms were among the mo...
Leishmania are obligated intracellular pathogens that develop almost exclusively in macrophages . Experimental leishmaniasis in mice is one of the most extensively studied models of intracellular infections both at the level of the parasite and host immune responses . We took advantage of Balb/c mice model to investiga...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "microarrays", "genomics", "molecular", "cell", "biology", "gene", "expression", "biology", "computational", "biology", "microbiology", "host-pathogen", "interaction", "molecular", "biology", "genetics", "and", "genomics" ]
2012
Transcriptomic Signature of Leishmania Infected Mice Macrophages: A Metabolic Point of View
Mycobacterium tuberculosis ( Mtb ) , possesses at least three type VII secretion systems , ESX-1 , -3 and -5 that are actively involved in pathogenesis and host-pathogen interaction . We recently showed that an attenuated Mtb vaccine candidate ( Mtb Δppe25-pe19 ) , which lacks the characteristic ESX-5-associated pe/ppe...
Mycobacterium tuberculosis ( Mtb ) , the causative agent of human tuberculosis , is one of the most widely spread human pathogens , responsible for more than 9 . 6 million of new tuberculosis cases and 1 . 5 million deaths , annually . The resurgence of pulmonary tuberculosis in immuno-compromised patients , including ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "blood", "cells", "t", "helper", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "immunology", "cell-mediated", "immunity", "cell", "differentiation", "vaccines", "preventive", "medicine", "developmental", "biology", "vaccina...
2016
CD4+ T Cells Recognizing PE/PPE Antigens Directly or via Cross Reactivity Are Protective against Pulmonary Mycobacterium tuberculosis Infection
The emergence and rapid global spread of the swine-origin H1N1/09 pandemic influenza A virus in humans underscores the importance of swine populations as reservoirs for genetically diverse influenza viruses with the potential to infect humans . However , despite their significance for animal and human health , relative...
Since 1998 , genetically and antigenically diverse influenza A viruses have circulated in North American swine due to continuous cross-species transmission and reassortment with avian and human influenza viruses , presenting a pandemic threat to humans . Millions of swine are transported year-round from the southern Un...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "infectious", "diseases", "biology", "computational", "biology", "evolutionary", "biology" ]
2011
Spatial Dynamics of Human-Origin H1 Influenza A Virus in North American Swine
Recent experiments showed that engineered Escherichia coli colonies grow and self-organize into periodic stripes with high and low cell densities in semi-solid agar . The stripes develop sequentially behind a radially propagating colony front , similar to the formation of many other periodic patterns in nature . These ...
One of the central problems in biology is to understand the underlying mechanisms responsible for spatial pattern formation in complex systems . This is a difficult task because the essential mechanisms for pattern formation often involve multiple space and time scales and are often buried in overwhelmingly complex phy...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "bacteriology", "cell", "physiology", "cell", "motility", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "engineering", "and", "technology", "pathogens", "signal", "processing", "microbiology", "biological", "locomotion", "simulatio...
2018
The role of intracellular signaling in the stripe formation in engineered Escherichia coli populations
Cancer is a heterogeneous disease often requiring a complexity of alterations to drive a normal cell to a malignancy and ultimately to a metastatic state . Certain genetic perturbations have been implicated for initiation and progression . However , to a great extent , underlying mechanisms often remain elusive . These...
Cancer is a complex disease with many subtypes that differ substantially with respect to their onset , progression , and response to treatment . Better understanding of the etiology and mechanism of cancer should help improve the diagnosis , prognosis , and treatment of cancer that will kill more than half a million Am...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computer", "science", "mathematics", "computational", "biology", "homo", "(human)", "genetics", "and", "genomics" ]
2008
Modeling Cancer Progression via Pathway Dependencies
The firing rate of single neurons in the mammalian hippocampus has been demonstrated to encode for a range of spatial and non-spatial stimuli . It has also been demonstrated that phase of firing , with respect to the theta oscillation that dominates the hippocampal EEG during stereotype learning behaviour , correlates ...
Changes in the strength of synaptic connections between neurons are believed to mediate processes of learning and memory in the brain . A computational theory of this synaptic plasticity was first provided by Donald Hebb within the context of a more general neural coding mechanism , whereby phase sequences of activity ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "neuroscience/cognitive", "neuroscience", "neuroscience/theoretical", "neuroscience", "computational", "biology/computational", "neuroscience" ]
2010
Dual Coding with STDP in a Spiking Recurrent Neural Network Model of the Hippocampus
Intrathecal antibody synthesis is a well-documented phenomenon in infectious neurological diseases as well as in demyelinating diseases , but little is known about the role of B cells in the central nervous systems . We examined B cell and T cell immunophenotypes in CSF of patients with HTLV-1-associated myelopathy/tro...
Regulation of the local immune response is crucial in protecting the central nervous system ( CNS ) from viral infection and immunopathologically mediated tissue damage . Intrathecal antibody synthesis is a well-documented phenomenon in infectious and demyelinating neurological diseases , but little is known about the ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "body", "fluids", "immune", "cells", "pathology", "and", "laboratory", "medicine", "immune", "physiology", "multiple", "sclerosis", "nervous", "system", "neurodegenerative", "diseases", "pathogens", "immunology"...
2018
Immunophenotypic characterization of CSF B cells in virus-associated neuroinflammatory diseases
Meiosis is a complex type of cell division that involves homologous chromosome pairing , synapsis , recombination , and segregation . When any of these processes is altered , cellular checkpoints arrest meiosis progression and induce cell elimination . Meiotic impairment is particularly frequent in organisms bearing ch...
Cells have different mechanisms to assess the proper occurrence of cellular events . These mechanisms are called checkpoints and are involved in the surveillance of processes such as DNA replication and cell division . A checkpoint at the pachytene stage arrests meiosis when defects in the process of homologous chromos...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "developmental", "biology/germ", "cells", "cell", "biology/cell", "growth", "and", "division", "genetics", "and", "genomics/chromosome", "biology", "evolutionary", "biology/nuclear", "structure", "and", "function" ]
2009
A High Incidence of Meiotic Silencing of Unsynapsed Chromatin Is Not Associated with Substantial Pachytene Loss in Heterozygous Male Mice Carrying Multiple Simple Robertsonian Translocations
When early canonical Wnt is experimentally inhibited , sea urchin embryos embody the concept of a Default Model in vivo because most of the ectodermal cell fates are specified as anterior neuroectoderm . Using this model , we describe here how the combination of orthogonally functioning anteroposterior Wnt and dorsoven...
The sea urchin embryo is similar to vertebrate embryos in that the default cell fate is potentially neurogenic , and normal development restricts the neural fate to the narrow area that locates at the anterior/dorsal region of the embryo . Because maintaining the default neural fate to the anterior/dorsal region is req...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "invertebrates", "neuronal", "differentiation", "neuroscience", "animals", "cell", "differentiation", "blastulas", "animal", "models", "developmental", "biology", "model", "organisms", "embryos", "echinoderms", "research", "and", "analysis", "methods", "ectoderm", "embryolo...
2016
Cooperative Wnt-Nodal Signals Regulate the Patterning of Anterior Neuroectoderm
The conundrum of cooperation has received increasing attention during the last decade . In this quest , the role of altruistic punishment has been identified as a mechanism promoting cooperation . Here we investigate the role of altruistic punishment on the emergence and maintenance of cooperation in structured populat...
Altruistic punishment — when a cooperative individual pays a cost to punish her defective partner — has been described as one of the mechanisms that help to explain cooperation's ubiquity in nature . Here , we investigate a model population where individuals interact with each other along the links of a network . The n...
[ "Abstract", "Introduction", "Model", "Results/Discussion" ]
[ "computer", "science", "stochastic", "processes", "computer", "modeling", "mathematics", "evolutionary", "biology", "population", "modeling", "evolutionary", "modeling", "biology", "computational", "biology", "evolutionary", "processes", "probability", "theory" ]
2013
Reward from Punishment Does Not Emerge at All Costs
Fascioliasis has been sporadically associated with chronic liver disease on previous studies . In order to describe the current evidence , we carried out a systematic review to assess the association between fascioliasis with liver fibrosis , cirrhosis and cancer . A systematic search of electronic databases ( PubMed ,...
Fascioliasis is a neglected infectious disease caused by the trematode Fasciola . This parasite ( liver fluke ) is endemic in many parts of the world including countries from Asia , Africa , Europe and the Americas . High prevalence rates of fascioliasis has been repeatedly reported in the Andean region of South Americ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "helminths", "tropical", "diseases", "fibrosis", "fascioliasis", "database", "searching", "parasitic", "diseases", "animals", "liver", "diseases", "trematodes", "developmental", "biology", "gastroenterology", "and", ...
2016
Association of Fasciola hepatica Infection with Liver Fibrosis, Cirrhosis, and Cancer: A Systematic Review
No vaccine is currently available for dengue virus ( DENV ) , therefore control programmes usually focus on managing mosquito vector populations . Entomological surveys provide the most common means of characterising vector populations and predicting the risk of local dengue virus transmission . Despite Indonesia being...
Geographically and economically , Indonesia is one of the most prominent countries in SE Asia , yet many of its endemic infectious diseases are poorly managed , controlled and understood . This includes dengue virus ( DENV ) , which can result in serious human disease and is transmitted by mosquitoes . Dengue risk asse...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "insect", "metamorphosis", "geographical", "locations", "animals", "indonesia", "java", "age", "groups", "developmental", "biology", "adults", "pupae", "infectious", "disease", "control", "insect", "vectors", "zo...
2016
Dengue in Java, Indonesia: Relevance of Mosquito Indices as Risk Predictors
Fusarium fujikuroi causes bakanae ( “foolish seedling” ) disease of rice which is characterized by hyper-elongation of seedlings resulting from production of gibberellic acids ( GAs ) by the fungus . This plant pathogen is also known for production of harmful mycotoxins , such as fusarins , fusaric acid , apicidin F an...
Fusarium fujikuroi causes bakanae disease of rice . Infected seedlings appear to be taller and more slender when compared to healthy seedlings due to its ability to produce gibberellic acids ( GAs ) . The disease is responsible for high yield losses , and its incidence varies with regions , rice cultivars grown and the...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Material", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "fusarium", "pathology", "and", "laboratory", "medicine", "pathogens", "microbiology", "hormones", "plant", "science", "rice", "plant", "hormones", "experimental", "organism", "systems", "seedlings", "molecular", "biology", "tech...
2017
Comparative genomics of geographically distant Fusarium fujikuroi isolates revealed two distinct pathotypes correlating with secondary metabolite profiles
During the first meiotic prophase , programmed DNA double-strand breaks ( DSBs ) are distributed non randomly at hotspots along chromosomes , to initiate recombination . In all organisms , more DSBs are formed than crossovers ( CO ) , the repair product that creates a physical link between homologs and allows their cor...
For sexual reproduction , meiosis is an essential step ensuring the formation of haploid gametes from diploid precursors of the germline . This reduction in the genome's content is achieved through a specialized type of division , where a single round of DNA replication is followed by two successive rounds of chromosom...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology" ]
2013
Differential Association of the Conserved SUMO Ligase Zip3 with Meiotic Double-Strand Break Sites Reveals Regional Variations in the Outcome of Meiotic Recombination
Schistosomiasis is endemic to many regions of the world and affects approximately 200 million people . Conventional adaptive T cell responses are considered to be the primary contributors to the pathogenesis of Schistosoma japonicum infection , leading to liver granuloma and fibrosis . However , the functional polariza...
Schistosomiasis is a global health concern that affects primarily tropical and subtropical areas . During a schistosome infection , the eggs are trapped in the host liver and products derived from eggs induce a polarized Th2 response , resulting in granuloma formation and eventually fibrosis . Thus , it is important to...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "blood", "cells", "innate", "immune", "system", "medicine", "and", "health", "sciences", "immune", "cells", "immune", "physiology", "cytokines", "granulomas", "immunology", "tropical", "diseases", "parasitic", "diseases", "liver", "diseases", "animal", "models", "immu...
2018
Toll-like receptor-2 regulates macrophage polarization induced by excretory-secretory antigens from Schistosoma japonicum eggs and promotes liver pathology in murine schistosomiasis
Malaria parasites have been shown to adjust their life history traits to changing environmental conditions . Parasite relapses and recrudescences—marked increases in blood parasite numbers following a period when the parasite was either absent or present at very low levels in the blood , respectively—are expected to be...
Seasonal fluctuations in the environment affect dramatically the abundance of insect species . These fluctuations have important consequences for the transmission of vector-borne diseases . Here we contend that malaria parasites may have evolved plastic transmission strategies as an adaptation to the fluctuations in mo...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "evolutionary", "ecology", "ecology", "and", "environmental", "sciences", "medicine", "and", "health", "sciences", "infectious", "disease", "epidemiology", "population", "dynamics", "microbiology", "parasitic", "diseases", "plant", "science", "plant", "pathology", "theore...
2014
Evolution of Plastic Transmission Strategies in Avian Malaria
Mutations in peroxin ( PEX ) genes lead to loss of peroxisomes , resulting in the formation of peroxisomal biogenesis disorders ( PBDs ) and early lethality . Studying PBDs and their animal models has greatly contributed to our current knowledge about peroxisomal functions . Very-long-chain fatty acid ( VLCFA ) accumul...
Peroxisomes are organelles that contain several enzymes and fatty acids required for many metabolic tasks in the cell , and upon peroxisome loss , their educts accumulate . One example is the accumulation of very-long-chain fatty acids ( VLCFAs ) with a chain length of more than 20 carbons . These fatty acids cannot be...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "lipolysis", "medicine", "and", "health", "sciences", "plant", "products", "diet", "developmental", "biology", "nutrition", "mitochondria", "bioenergetics", "peroxisomes", "cellular", "structures", "and", "organelles", "lipids", "crop", "science", "life", "cycles", "che...
2018
Dietary rescue of lipotoxicity-induced mitochondrial damage in Peroxin19 mutants
Understanding how hepatitis C virus ( HCV ) induces and circumvents the host's natural killer ( NK ) cell-mediated immunity is of critical importance in efforts to design effective therapeutics . We report here the decreased expression of the NKG2D activating receptor as a novel strategy adopted by HCV to evade NK-cell...
Natural killer ( NK ) cells are part of the innate immune response against virus infection . Their activation is the net result of signals emanating from a panel of inhibitory and activating receptors , among which the NKG2D activating receptor plays a major role . NKG2D ligands , the MHC class I related Chain ( MIC ) ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunology/immune", "response", "immunology/innate", "immunity", "immunology/immunity", "to", "infections" ]
2010
Hepatitis C Virus (HCV) Evades NKG2D-Dependent NK Cell Responses through NS5A-Mediated Imbalance of Inflammatory Cytokines
When visual contrast changes , retinal ganglion cells adapt by adjusting their sensitivity as well as their temporal filtering characteristics . The latter has classically been described by contrast-induced gain changes that depend on temporal frequency . Here , we explored a new perspective on contrast-induced changes...
Our sensory systems have to process stimuli under a wide range of environmental conditions . To cope with this challenge , the involved neurons adapt by adjusting their signal processing to the recently encountered intensity range . In the visual system , one finds , for example , that higher visual contrast leads to c...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[]
2015
Spike-Triggered Covariance Analysis Reveals Phenomenological Diversity of Contrast Adaptation in the Retina
Leishmania ( L . ) killicki ( syn . L . tropica ) , which causes cutaneous leishmaniasis in Maghreb , was recently described in this region and identified as a subpopulation of L . tropica . The present genetic analysis was conducted to explore the spatio-temporal distribution of L . killicki ( syn . L . tropica ) and ...
Leishmania killicki ( syn . L . tropica ) was discovered in 1986 . Few studies have been conducted on this parasite exclusively described in Maghreb . Consequently , many elements on its epidemiology , transmission , population structure and dynamics remain unknown . To better understand the evolution of this parasite ...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2015
Comparison of Leishmania killicki (syn. L. tropica) and Leishmania tropica Population Structure in Maghreb by Microsatellite Typing
Changes in climate and environmental conditions could be the driving factors for the transmission of hantavirus . Thus , a thorough collection and analysis of data related to the epidemic status of hemorrhagic fever with renal syndrome ( HFRS ) and the association between HFRS incidence and meteorological factors , suc...
China has the largest number of hemorrhagic fever with renal syndrome ( HFRS ) cases in the world . With the acceleration of China’s urbanization process , especially in the process of rapid transition of China’s agriculture-related landscapes to urban landscapes , the dual role of climate change and environmental chan...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "spring", "statistics", "china", "atmospheric", "science", "geographical", "locations", "seasons", "hemorrhagic", "fever", "with", "renal", "syndrome", "mathematics", "research", "and", "analysis", "methods", "climate", "change",...
2019
Distribution of geographical scale, data aggregation unit and period in the correlation analysis between temperature and incidence of HFRS in mainland China: A systematic review of 27 ecological studies
Nod-like receptors ( NLRs ) comprise a large family of intracellular pattern- recognition receptors . Members of the NLR family assemble into large multiprotein complexes , termed the inflammasomes . The NLR family , pyrin domain-containing 3 ( NLRP3 ) is triggered by a diverse set of molecules and signals , and forms ...
The innate immune system , the first line of defense against invading pathogens , plays a key role not only in limiting microbe replications at early stages of infection , but also in initiating and orchestrating antigen-specific adaptive immune responses . The innate immune responses against viruses usually rely on re...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "immunity", "innate", "immunity", "immunology", "biology" ]
2012
Encephalomyocarditis Virus Viroporin 2B Activates NLRP3 Inflammasome
Computational methods for predicting evolutionarily conserved rather than thermodynamic RNA structures have recently attracted increased interest . These methods are indispensable not only for elucidating the regulatory roles of known RNA transcripts , but also for predicting RNA genes . It has been notoriously difficu...
Not only is the prediction of evolutionarily conserved RNA structures important for elucidating the potential functions of RNA sequences and the mechanisms by which these functions are exerted , but it also lies at the core of RNA gene prediction . To get an accurate prediction of the conserved RNA structure , we need ...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "evolutionary", "biology", "genetics", "and", "genomics", "vertebrates", "computational", "biology" ]
2007
SimulFold: Simultaneously Inferring RNA Structures Including Pseudoknots, Alignments, and Trees Using a Bayesian MCMC Framework
Biophysical models of cardiac tension development provide a succinct representation of our understanding of force generation in the heart . The link between protein kinetics and interactions that gives rise to high cooperativity is not yet fully explained from experiments or previous biophysical models . We propose a b...
Force generation in cardiac muscle cells is driven by changes in calcium concentration . Relatively small changes in the calcium concentration over the course of a heart beat lead to the large changes in force required to fully contract and relax the heart . This is known as ‘cooperative activation’ , and involves a co...
[ "Abstract", "Introduction", "Models", "Results", "Discussion" ]
[]
2015
A Spatially Detailed Model of Isometric Contraction Based on Competitive Binding of Troponin I Explains Cooperative Interactions between Tropomyosin and Crossbridges
The genus Henipavirus in the family Paramyxoviridae contains two viruses , Hendra virus ( HeV ) and Nipah virus ( NiV ) for which pteropid bats act as the main natural reservoir . Each virus also causes serious and commonly lethal infection of people as well as various species of domestic animals , however little is kn...
Hendra and Nipah viruses are 2 highly pathogenic paramyxoviruses that have emerged from bats within the last two decades . Both are capable of causing fatal disease in both humans and many mammal species . Serological and molecular evidence for henipa-like viruses have been reported from numerous locations including As...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "veterinary", "diseases", "emerging", "infectious", "diseases", "virology", "emerging", "viral", "diseases", "veterinary", "virology", "biology", "microbiology", "veterinary", "science" ]
2012
Cedar Virus: A Novel Henipavirus Isolated from Australian Bats
Zygotic gene expression programs control cell differentiation in vertebrate development . In Xenopus , these programs are initiated by local induction of regulatory genes through maternal signaling activities in the wake of zygotic genome activation ( ZGA ) at the midblastula transition ( MBT ) . These programs lay dow...
Brahma-related-gene-1 ( Brg1 ) is a catalytic subunit of mammalian SWI/SNF chromatin remodeling complexes . Loss of maternal Brg1 protein arrests development in mice at the 2-cell stage , while null homozygotes die at the blastocyst stage . These early requirements have precluded any analysis of Brg1’s embryonic functi...
[ "Abstract", "Introduction", "Results", "Discussion", "Conclusion", "Methods" ]
[ "morpholino", "rna", "interference", "gene", "regulation", "messenger", "rna", "vertebrates", "nucleotides", "animals", "xenopus", "blastulas", "animal", "models", "dna", "transcription", "developmental", "biology", "model", "organisms", "amphibians", "experimental", "org...
2017
Brg1 chromatin remodeling ATPase balances germ layer patterning by amplifying the transcriptional burst at midblastula transition
The mechanisms of evolution of plant viruses are being unraveled , yet the timescale of their evolution remains an enigma . To address this critical issue , the divergence time of plant viruses at the intra- and inter-specific levels was assessed . The time of the most recent common ancestor ( TMRCA ) of Rice yellow mo...
The timescale of the evolution of plant viruses is an enigma , and even its order of magnitude is unknown . This critical issue is addressed here by calculating the age of plant viruses . An accurate estimate of the age of Rice yellow mottle virus ( RYMV ) was obtained by statistical analysis of a set of dated sequence...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "virology/emerging", "viral", "diseases", "virology/virus", "evolution", "and", "symbiosis", "evolutionary", "biology/evolutionary", "ecology" ]
2008
Diversification of Rice Yellow Mottle Virus and Related Viruses Spans the History of Agriculture from the Neolithic to the Present
The olfactory ( OR ) and vomeronasal receptor ( VR ) repertoires are collectively encoded by 1700 genes and pseudogenes in the mouse genome . Most OR and VR genes were identified by comparative genomic techniques and therefore , in many of those cases , only their protein coding sequences are defined . Some also lack e...
The sense of smell in mice involves the detection of odors and pheromones by many hundreds of olfactory and vomeronasal receptors . The genes that encode these receptors account for around 5% of the whole gene catalog , but they are poorly understood because they are very similar to each other , and are thought to be t...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome", "expression", "analysis", "neuroscience", "animal", "models", "model", "organisms", "genome", "analysis", "bioassays", "and", "physiological", "analysis", "molecular", "genetics", "research", "and", "analysis", "methods", "gene", "expression", "mouse", "models...
2014
The Olfactory Transcriptomes of Mice
Host restriction factors constitute a formidable barrier for viral replication to which many viruses have evolved counter-measures . Human SAMD9 , a tumor suppressor and a restriction factor for poxviruses in cell lines , is antagonized by two classes of poxvirus proteins , represented by vaccinia virus ( VACV ) K1 and...
Zoonotic viral infections represent a major threat to public health . For many viruses , host species-specific difference in viral entry receptors presents a major hurdle for cross-species transmission . Poxviruses , however , can enter nearly any animal cell . Why many poxviruses show strict host species specificity a...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "medicine", "and", "health", "sciences", "poxviruses", "pathology", "and", "laboratory", "medicine", "hela", "cells", "pathogens", "biological", "cultures", "microbiology", "viruses", "immunoprecipitation", "dna", "viruses", "cell", "cultures", "research", "and", "analy...
2018
A paralogous pair of mammalian host restriction factors form a critical host barrier against poxvirus infection
Wolbachia are maternally transmitted intracellular bacterial symbionts that infect approximately 40% of all insect species . Though several strains of Wolbachia naturally infect Drosophila melanogaster and provide resistance against viral pathogens , or provision metabolites during periods of nutritional stress , one v...
Wolbachia are obligate intracellular , symbiotic bacteria that infect approximately 40% of insect species , as well as filarial nematodes , arachnids and terrestrial isopods . While the vast majority of Wolbachia strains impose few fitness costs to their host , one strain wMelPop is unique as it lacks the ability to re...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "invertebrates", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "microbiology", "invertebrate", "genomics", "animals", "wolbachia", "animal", "models", "bacterial", "diseases", "developmental", "biology", "drosophila", "melanogaster",...
2016
Intensity of Mutualism Breakdown Is Determined by Temperature Not Amplification of Wolbachia Genes
Proline–tyrosine nuclear localization signals ( PY-NLSs ) are recognized and transported into the nucleus by human Karyopherin ( Kap ) β2/Transportin and yeast Kap104p . Multipartite PY-NLSs are highly diverse in sequence and structure , share a common C-terminal R/H/KX2–5PY motif , and can be subdivided into hydrophob...
To travel between the cytoplasm and nucleus , proteins rely on a family of transport proteins known as the karyopherinβ family . Karyopherinβ2 , the human version of a family member , recognizes cargo proteins containing a class of nuclear localization signal known as the PY-NLS . The yeast homolog of Karyopherinβ2 , K...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "biophysics", "biochemistry" ]
2008
Modular Organization and Combinatorial Energetics of Proline–Tyrosine Nuclear Localization Signals
One of the most intriguing questions in evolution is how organisms exhibit suitable phenotypic variation to rapidly adapt in novel selective environments . Such variability is crucial for evolvability , but poorly understood . In particular , how can natural selection favour developmental organisations that facilitate ...
A striking feature of evolving organisms is their ability to acquire novel characteristics that help them adapt in new environments . The origin and the conditions of such ability remain elusive and is a long-standing question in evolutionary biology . Recent theory suggests that organisms can evolve designs that help ...
[ "Abstract", "Introduction", "Results", "and", "discussion", "Methods" ]
[ "learning", "organismal", "evolution", "acoustics", "social", "sciences", "neuroscience", "learning", "and", "memory", "developmental", "biology", "cognitive", "psychology", "sound", "pressure", "evolutionary", "adaptation", "evolutionary", "genetics", "physics", "psycholog...
2017
How evolution learns to generalise: Using the principles of learning theory to understand the evolution of developmental organisation
Pathogenic bacterial infections of the lung are life threatening and underpin chronic lung diseases . Current treatments are often ineffective potentially due to increasing antibiotic resistance and impairment of innate immunity by disease processes and steroid therapy . Manipulation miRNA directly regulating anti-micr...
MicroRNAs regulate pathogen recognition pathways by modulating translation . In the immune system , miRNAs have been identified as important regulators of gene expression programs , which regulate differentiation , growth and function of innate and adaptive immune cells . Using miRNA microarray , we demonstrated that l...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Antagonism of miR-328 Increases the Antimicrobial Function of Macrophages and Neutrophils and Rapid Clearance of Non-typeable Haemophilus Influenzae (NTHi) from Infected Lung
Seminal fluid proteins transferred from males to females during copulation are required for full fertility and can exert dramatic effects on female physiology and behavior . In Drosophila melanogaster , the seminal protein sex peptide ( SP ) affects mated females by increasing egg production and decreasing receptivity ...
Reproduction requires more than a sperm and an egg . In animals with internal fertilization , other proteins in the seminal fluid and the female are essential for full fertility . Although hundreds of such reproductive proteins are known , our ability to understand how they interact remains limited . In this study , we...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "animal", "models", "drosophila", "melanogaster", "model", "organisms", "genetics", "comparative", "genomics", "biology", "genomics", "evolutionary", "biology", "genomic", "evolution" ]
2014
Evolutionary Rate Covariation Identifies New Members of a Protein Network Required for Drosophila melanogaster Female Post-Mating Responses
Parasitic zoonoses ( PZs ) pose a significant but often neglected threat to public health , especially in developing countries . In order to obtain a better understanding of their health impact , summary measures of population health may be calculated , such as the Disability-Adjusted Life Year ( DALY ) . However , the...
Various parasites that infect humans require animals in some stage of their life cycle . Infection with these so-called zoonotic parasites may vary from asymptomatic carriership to long-term morbidity and even death . Although data are still scarce , it is clear that parasitic zoonoses ( PZs ) present a significant bur...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[]
2014
The Burden of Parasitic Zoonoses in Nepal: A Systematic Review
Leishmaniases are neglected parasitic diseases in spite of the major burden they inflict on public health . The identification of novel drugs and targets constitutes a research priority . For that purpose we used Leishmania infantum initiation factor 4A ( LieIF ) , an essential translation initiation factor that belong...
Leishmaniases constitute a group of neglected parasitic diseases that inflict major burden on public health . Novel drugs and targets need to be identified since current therapies have adverse side effects . Herein , we focused on Leishmania infantum translation initiation factor 4A ( LieIF ) , as a potential drug targ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "blood", "cells", "medicine", "and", "health", "sciences", "immune", "cells", "enzymes", "immunology", "enzymology", "microbiology", "parasitic", "diseases", "protozoan", "life", "cycles", "parasitic", "protozoans", "phosphatases", "developmental", "biology", "protozoans"...
2018
Identification of novel leishmanicidal molecules by virtual and biochemical screenings targeting Leishmania eukaryotic translation initiation factor 4A
Biofilms are communities of bacteria that grow encased in an extracellular matrix that often contains proteins . The spatial organization and the molecular interactions between matrix scaffold proteins remain in most cases largely unknown . Here , we report that Bap protein of Staphylococcus aureus self-assembles into ...
Major components of the biofilm matrix scaffold are proteins that assemble to create a unified structure that maintain bacteria attached to each other and to surfaces . We provide evidence that a surface protein present in several staphylococcal species forms functional amyloid aggregates to build the biofilm matrix in...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "biofilms", "cell", "walls", "medicine", "and", "health", "sciences", "fluorescence", "pathology", "and", "laboratory", "medicine", "enzymes", "pathogens", "microbiology", "enzymology", "staphylococcus", "aureus", "electromagnetic", "radiation", "cellular", ...
2016
Staphylococcal Bap Proteins Build Amyloid Scaffold Biofilm Matrices in Response to Environmental Signals
Guiding axon growth cones towards their targets is a fundamental process that occurs in a developing nervous system . Several major signaling systems are involved in axon-guidance , and disruption of these systems causes axon-guidance defects . However , the specific role of the environment in which axons navigate in r...
During nervous system development , once formed from neuroblasts , neurons grow axons in the direction of their synaptic partners . Genetic forces guide these axon growth cones towards the target . This is known as axon guidance or pathfinding . There are a number of proteins that regulate axon-pathfinding . The well-k...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
The Midline Protein Regulates Axon Guidance by Blocking the Reiteration of Neuroblast Rows within the Drosophila Ventral Nerve Cord
Multipotent neural crest ( NC ) progenitors generate an astonishing array of derivatives , including neuronal , skeletal components and pigment cells ( chromatophores ) , but the molecular mechanisms allowing balanced selection of each fate remain unknown . In zebrafish , melanocytes , iridophores and xanthophores , th...
Multipotent neural crest ( NC ) progenitors generate an astonishing array of derivatives , including neuronal , skeletal components and pigment cells , but the molecular mechanisms allowing balanced selection of each fate remain unknown . In zebrafish , melanocytes , iridophores and xanthophores , the three chromatopho...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "fish", "vertebrates", "pigments", "animals", "cell", "differentiation", "epithelial", "cells", "simulation", "and", "modeling", "animal", "models", "osteichthyes", "developmental", "biology", "model", "organisms", "stem", "cells...
2018
A systems biology approach uncovers the core gene regulatory network governing iridophore fate choice from the neural crest
One key problem in precision genome editing is the unpredictable plurality of sequence outcomes at the site of targeted DNA double stranded breaks ( DSBs ) . This is due to the typical activation of the versatile Non-homologous End Joining ( NHEJ ) pathway . Such unpredictability limits the utility of somatic gene edit...
New gene editing tools precisely break DNA at pre-defined genomic locations , but cells repair these lesions using diverse pathways that often lead to unpredictable outcomes in the resulting DNA sequences . This sequence diversity in gene editing outcomes represents an important obstacle to the application of this tech...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "biotechnology", "genome", "engineering", "fish", "engineering", "and", "technology", "nucleases", "enzymes", "synthetic", "biology", "dna-binding", "proteins", "vertebrates", "cloning", "enzymology", "microhomology-mediated", "end", "joining", "animals", "synthetic", "bioe...
2018
Robust activation of microhomology-mediated end joining for precision gene editing applications
Brain metastasis ( BM ) is a major complication of lung adenocarcinoma ( LAD ) . An investigation of the pathogenic mechanisms of BM , as well as the identification of appropriate molecular markers , is necessary . The aim of this study was to determine the expression patterns of microRNAs ( miRNAs ) in LAD with BM , a...
Brain metastasis ( BM ) is a major complication of lung carcinoma . Here , we aimed to identify the key miRNAs involved in BM lung cancer . We first profiled miRNA expression in 32 tissues from NSCLC patients with BM and 55 tissues from NSCLC patients without BM . We independently validated our results in 68 additional...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "transfection", "medicine", "and", "health", "sciences", "luciferase", "diagnostic", "radiology", "natural", "antisense", "transcripts", "gene", "regulation", "enzymes", "cancers", "and", "neoplasms", "enzymology", "basic", "cancer", "research", "in", "vivo", "imaging",...
2019
Molecular predictors of brain metastasis-related microRNAs in lung adenocarcinoma
Tissue-specific gene expression plays a fundamental role in metazoan biology and is an important aspect of many complex diseases . Nevertheless , an organism-wide map of tissue-specific expression remains elusive due to difficulty in obtaining these data experimentally . Here , we leveraged existing whole-animal Caenor...
In animals , a crucial facet of any gene's function is the tissue or cell type in which that gene is expressed and the proteins that it interacts with in that cell . However , genome-wide identification of expression across the multitude of tissues of varying size and complexity is difficult to achieve experimentally ....
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "computational", "biology/sequence", "motif", "analysis", "genetics", "and", "genomics/functional", "genomics", "genetics", "and", "genomics/gene", "expression", "computational", "biology/transcriptional", "regulation", "developmental", "biology/aging" ]
2009
Global Prediction of Tissue-Specific Gene Expression and Context-Dependent Gene Networks in Caenorhabditis elegans
Dengue disease is currently a major health problem in Indonesia and affects all provinces in the country , including Semarang Municipality , Central Java province . While dengue is endemic in this region , only limited data on the disease epidemiology is available . To understand the dynamics of dengue in Semarang , we...
We studied dengue disease in Semarang municipality , Central Java , one of the endemic regions in Indonesia . The disease occurred in wide geographical regions which include urban , rural , coastal , and highland areas . All four dengue virus serotypes were found . The infecting serotypes were not associated with disea...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "molecular", "epidemiology", "epidemiology", "dengue", "fever", "neglected", "tropical", "diseases", "dengue", "biology", "viral", "diseases", "evolutionary", "biology", "genomic", "evolution" ]
2013
Molecular Surveillance of Dengue in Semarang, Indonesia Revealed the Circulation of an Old Genotype of Dengue Virus Serotype-1
We consider the problem of genetic association testing of a binary trait in a sample that contains related individuals , where we adjust for relevant covariates and allow for missing data . We propose CERAMIC , an estimating equation approach that can be viewed as a hybrid of logistic regression and linear mixed-effect...
Case-control association testing has proven to be useful for identification of genetic variants that affect susceptibility to disease . One can expect to gain power for detecting such variants by including relevant covariates in the analysis , by accounting for any relatedness of sampled individuals , and by making use...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "genome-wide", "association", "studies", "medicine", "and", "health", "sciences", "cardiovascular", "anatomy", "variant", "genotypes", "genetic", "mapping", "simulation", "and", "modeling", "diabetes", "mellitus", "endocrine", "disorders", "mathematics", "statistics", "(m...
2016
CERAMIC: Case-Control Association Testing in Samples with Related Individuals, Based on Retrospective Mixed Model Analysis with Adjustment for Covariates
In line with the key role of methionine in protein biosynthesis initiation and many cellular processes most microorganisms have evolved mechanisms to synthesize methionine de novo . Here we demonstrate that , in the bacterial pathogen Staphylococcus aureus , a rare combination of stringent response-controlled CodY acti...
Prokaryote metabolism is key for our understanding of bacterial virulence and pathogenesis and it is also an area with huge opportunity to identify novel targets for antibiotic drugs . Here , we have addressed the so far poorly characterized regulation of methionine biosynthesis in S . aureus . We demonstrate that meth...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "bacteriology", "bacterial", "physiology", "microbial", "metabolism", "gene", "expression", "gene", "regulation", "genetics", "molecular", "genetics", "staphylococci", "biology", "microbiology", "bacterial", "pathogens", "gram", "positive" ]
2013
Methionine Biosynthesis in Staphylococcus aureus Is Tightly Controlled by a Hierarchical Network Involving an Initiator tRNA-Specific T-box Riboswitch
A growing number of solved protein structures display an elongated structural domain , denoted here as alpha-rod , composed of stacked pairs of anti-parallel alpha-helices . Alpha-rods are flexible and expose a large surface , which makes them suitable for protein interaction . Although most likely originating by tande...
Many proteins have an elongated structural domain formed by a stack of alpha helices ( alpha-rod ) , often found to interact with other proteins . The identification of an alpha-rod in a protein can therefore tell something about both the function and the structure of that protein . Though alpha-rods can be readily ide...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "molecular", "biology/bioinformatics", "computational", "biology/protein", "structure", "prediction", "computational", "biology/macromolecular", "sequence", "analysis", "biochemistry/macromolecular", "assemblies", "and", "machines" ]
2009
Detection of Alpha-Rod Protein Repeats Using a Neural Network and Application to Huntingtin
RNase H enzymes promote genetic stability by degrading aberrant RNA∶DNA hybrids and by removing ribonucleotide monophosphates ( rNMPs ) that are present in duplex DNA . Here , we report that loss of RNase H2 in yeast is associated with mutations that extend identity between the arms of imperfect inverted repeats ( quas...
Mutation rates are correlated with the level of gene expression in budding yeast , demonstrating a link between transcription and stability of the underlying DNA template . In the current work , we describe a novel type of transcription-associated mutation that converts imperfect inverted repeats ( quasi-palindromes or...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2013
RNA∶DNA Hybrids Initiate Quasi-Palindrome-Associated Mutations in Highly Transcribed Yeast DNA
Toll-like receptors ( TLRs ) are important regulators of the innate immune response to pathogens , including Mycobacterium leprae , which is recognized by TLR1/2 heterodimers . We previously identified a transmembrane domain polymorphism , TLR1_T1805G , that encodes an isoleucine to serine substitution and is associate...
Mycobacterium leprae ( ML ) causes a disabling and stigmatizing disease that is characterized by distinct immune responses . ML produces a spectrum of illness in humans , and several lines of evidence indicate that host genetic factors influence susceptibility and clinical manifestations . Leprosy can occur as the lepr...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "infectious", "diseases/neglected", "tropical", "diseases", "immunology/innate", "immunity", "genetics", "and", "genomics/genetics", "of", "the", "immune", "system" ]
2008
Human TLR1 Deficiency Is Associated with Impaired Mycobacterial Signaling and Protection from Leprosy Reversal Reaction
Muscle contractions are generated by cyclical interactions of myosin heads with actin filaments to form the actomyosin complex . To simulate actomyosin complex stable states , mathematical models usually define an energy landscape with a corresponding number of wells . The jumps between these wells are defined through ...
Mathematical models are of fundamental importance in the quantitative verification of biological hypotheses . Muscle contraction models assume the existence of several stable states for the myosin head , whereas the transition rates between states are defined to fit experimental data . The ratio of the forward and back...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "and", "Discussion" ]
[ "cell", "motility", "medicine", "and", "health", "sciences", "actin", "filaments", "myofibrils", "classical", "mechanics", "muscle", "tissue", "cardiovascular", "anatomy", "mathematical", "models", "muscle", "contraction", "mechanical", "energy", "molecular", "motors", ...
2016
Including Thermal Fluctuations in Actomyosin Stable States Increases the Predicted Force per Motor and Macroscopic Efficiency in Muscle Modelling
Epilepsy is a common disabling disease with complex , multifactorial genetic and environmental etiology . The small fraction of epilepsies subject to Mendelian inheritance offers key insight into epilepsy disease mechanisms; and pathologies brought on by mutations in a single gene can point the way to generalizable the...
Epilepsy is a common disabling disorder characterized by seizures with complex genetic and environmental components . The absence of a definitive pathophysiology for epilepsy stymies the development of effective treatment strategies . In a small fraction of epilepsy cases however , single gene mutations may illuminate ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Seizures Are Regulated by Ubiquitin-specific Peptidase 9 X-linked (USP9X), a De-Ubiquitinase
The capacity of organisms to tune their development in response to environmental cues is pervasive in nature . This phenotypic plasticity is particularly striking in plants , enabled by their modular and continuous development . A good example is the activation of lateral shoot branches in Arabidopsis , which develop f...
Many organisms adjust their development depending on environmental conditions . This is particularly striking in plants , with development constantly tuned throughout their lives . A good example is the modulation of shoot branching in response to external environmental cues . We characterised hundreds of genetically d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome-wide", "association", "studies", "chemical", "compounds", "quantitative", "trait", "loci", "population", "genetics", "brassica", "nitrates", "model", "organisms", "experimental", "organism", "systems", "genome", "analysis", "population", "biology", "plants", "flow...
2019
Natural variation in Arabidopsis shoot branching plasticity in response to nitrate supply affects fitness
Hypoxic microenvironments are generated during fungal infection . It has been described that to survive in the human host , fungi must also tolerate and overcome in vivo microenvironmental stress conditions including low oxygen tension; however nothing is known how Paracoccidioides species respond to hypoxia . The genu...
The genus Paracoccidioides is composed of species that are causative agents of paracoccidioidomycosis ( PCM ) , a neglected human granulomatous mycosis , endemic in Latin America . To survive in the human host , fungi must tolerate and overcome in vivo micro environmental stress conditions , including low oxygen levels...
[ "Abstract", "Introduction", "Methods", "Results", "and", "Discussion" ]
[]
2015
Characterization of the Paracoccidioides Hypoxia Response Reveals New Insights into Pathogenesis Mechanisms of This Important Human Pathogenic Fungus
Multistability and scale-invariant fluctuations occur in a wide variety of biological organisms from bacteria to humans as well as financial , chemical and complex physical systems . Multistability refers to noise driven switches between multiple weakly stable states . Scale-invariant fluctuations arise when there is a...
Biological systems are able to adapt to rapidly and widely changing environments . Many biological organisms employ two distinct mechanisms that improve their survival in these circumstances: Firstly they exhibit rapid , qualitative changes in their internal dynamics; secondly they possess the ability to respond to cha...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "complex", "systems", "mathematics", "biophysic", "al", "simulations", "computational", "neuroscience", "applied", "mathematics", "biology", "computational", "biology" ]
2012
A Canonical Model of Multistability and Scale-Invariance in Biological Systems
Germline mutations in the adenomatous polyposis coli ( APC ) gene are responsible for familial adenomatous polyposis ( FAP ) , an autosomal dominant hereditary predisposition to the development of multiple colorectal adenomas and of a broad spectrum of extra-intestinal tumors . Moreover , somatic APC mutations play a r...
Although signal transduction pathways are often described as “on–off” systems , the more quantitative aspects of signalling are likely to represent a very important means of regulation of the downstream biological outcomes . Mutations in members of the canonical Wnt signaling pathway represent among the most common def...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "oncology/breast", "cancer", "developmental", "biology/stem", "cells", "cell", "biology", "molecular", "biology", "genetics", "and", "genomics/cancer", "genetics", "pathology/molecular", "pathology" ]
2009
A Targeted Constitutive Mutation in the Apc Tumor Suppressor Gene Underlies Mammary But Not Intestinal Tumorigenesis
Variations on the statement “the variant surface glycoprotein ( VSG ) coat that covers the external face of the mammalian bloodstream form of Trypanosoma brucei acts a physical barrier” appear regularly in research articles and reviews . The concept of the impenetrable VSG coat is an attractive one , as it provides a c...
African trypanosomes have evolved two key strategies to prevent killing by the host immune response and , thus , maintain a long-term infection in a mammal . Both are based on a densely packed coat of a single protein , the variant surface glycoprotein ( VSG ) , which covers the entire extracellular surface of the cell...
[ "Abstract", "The", "VSG", "Coat", "The", "Roles", "of", "VSG", "N-linked", "Oligosaccharides", "Non-VSG", "Proteins", "Present", "in", "the", "VSG", "Coat", "Interactions", "with", "the", "Adaptive", "and", "Innate", "Immune", "System", "Investigation", "of", "H...
[]
2015
How Does the VSG Coat of Bloodstream Form African Trypanosomes Interact with External Proteins?
The Kato-Katz is the most common diagnostic method for Schistosoma mansoni infection . However , the day-to-day variability in host egg-excretion and its low detection sensitivity are major limits for its use in low transmission zones and after widespread chemotherapy . We evaluated the accuracy of circulating cathodic...
Recent momentum for the control of neglected tropical diseases has generated a renewed interest in the control of schistosomiasis , resulting in the increase of large scale mass drug administration of praziquantel in several countries . As a consequence , there is a need to develop more sensitive diagnostic tools , esp...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "test", "evaluation", "schistosomiasis", "public", "health", "and", "epidemiology", "diagnostic", "medicine", "epidemiology", "neglected", "tropical", "diseases", "epidemiological", "methods", "parasitic", "diseases" ]
2012
Evaluation of Circulating Cathodic Antigen (CCA) Urine-Tests for Diagnosis of Schistosoma mansoni Infection in Cameroon
Severe fever with thrombocytopenia syndrome virus ( SFTSV ) , an emerging human pathogen naturally transmitted by ticks , has spread widely since it was first detected in 2010 . Although SFTSV-specific antibodies have been detected in wild birds , these natural reservoir and amplifying hosts for the virus have not been...
Severe fever with thrombocytopenia syndrome virus ( SFTSV ) is an emerging human pathogen naturally transmitted by ticks . Our recent study has showed that some species of migratory birds , such as swan geese and spotted doves , can be parasitized by Haemaphysalis longicornis , an SFTSV vector , and antibodies against ...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "reverse", "transcriptase-polymerase", "chain", "reaction", "invertebrates", "medicine", "and", "health", "sciences", "immune", "physiology", "ixodes", "immunology", "social", "sciences", "vertebrates", "parasitic", "diseases", "animals", "euthanasia", "animal", "behavior",...
2019
Susceptibility of spotted doves (Streptopelia chinensis) to experimental infection with the severe fever with thrombocytopenia syndrome phlebovirus
Telomeres distinguish chromosome ends from double-strand breaks ( DSBs ) and prevent chromosome fusion . However , telomeres can also interfere with DNA repair , as shown by a deficiency in nonhomologous end joining ( NHEJ ) and an increase in large deletions at telomeric DSBs . The sensitivity of telomeric regions to ...
The ends of chromosomes , called telomeres , prevent chromosome ends from appearing as DNA double-strand breaks ( DSBs ) and prevent chromosome fusion by forming a specialized nucleo-protein complex . The critical function of telomeres in end protection has a downside , in that it interferes with the repair of DSBs tha...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biology" ]
2013
The Role of ATM in the Deficiency in Nonhomologous End-Joining near Telomeres in a Human Cancer Cell Line
Many meiotic systems in female animals include a lengthy arrest in G2 that separates the end of pachytene from nuclear envelope breakdown ( NEB ) . However , the mechanisms by which a meiotic cell can arrest for long periods of time ( decades in human females ) have remained a mystery . The Drosophila Matrimony ( Mtrm ...
Many meiotic systems in females animals include a lengthy arrest period ( spanning days in flies and to decades in humans ) that separates the early and late stages of meiosis . Such an arrest raises the question: how can the quiescent meiotic cell cycle be precisely awakened or re-started ? At least in principle , the...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods", "Supporting", "Information" ]
[ "cell", "biology", "genetics", "and", "genomics" ]
2007
The Inhibition of Polo Kinase by Matrimony Maintains G2 Arrest in the Meiotic Cell Cycle
Insect cuticle is composed primarily of chitin and structural proteins . To study the function of structural cuticular proteins , we focused on the proteins present in elytra ( modified forewings that become highly sclerotized and pigmented covers for the hindwings ) of the red flour beetle , Tribolium castaneum . We i...
Primitive insects have two pairs of membranous flight wings , but during the evolution of the beetle lineage the forewings lost their flight function and became modified as hard , rigid covers called elytra for protection of soft body parts of the abdomen and also the delicate flexible hindwings , which retained their ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "functional", "genomics", "rna", "interference", "gene", "function", "developmental", "biology", "molecular", "development", "proteins", "gene", "expression", "structural", "proteins", "biology", "extracellular", "matrix", "proteins", "morphogens", "biochemistry", "genetics...
2012
Formation of Rigid, Non-Flight Forewings (Elytra) of a Beetle Requires Two Major Cuticular Proteins
Scrub typhus , murine typhus , and leptospirosis are widely neglected infectious diseases caused by Orientia tsutsugamushi , Rickettsia typhi , and pathogenic Leptospira spp . , respectively . Patients usually present with non-specific symptoms and therefore are commonly diagnosed with acute undifferentiated febrile il...
Scrub typhus , murine typhus , and leptospirosis are diagnosed as acute undifferentiated febrile illness . Diagnostic tests for these diseases depend on antibody detection . However , antibody detection is still limited by its tendency to return negative results during the early phase of aforementioned diseases . In th...
[ "Abstract", "Introduction", "Materials", "and", "methods", "Results", "Discussion" ]
[ "typhus", "medicine", "and", "health", "sciences", "leptospira", "pathology", "and", "laboratory", "medicine", "pathogens", "tropical", "diseases", "microbiology", "organisms", "bacterial", "diseases", "neglected", "tropical", "diseases", "molecular", "biology", "techniqu...
2019
Development of multiplex PCR for neglected infectious diseases
Immune response against human cytomegalovirus ( HCMV ) includes a set of persistent cytotoxic NK and CD8 T cells devoted to eliminate infected cells and to prevent reactivation . CD8 T cells against HCMV antigens ( pp65 , IE1 ) presented by HLA class-I molecules are well characterized and they associate with efficient ...
Understanding the mechanisms of immune control of viral infection is crucial to improve diagnosis and to design efficient immunotherapies . CD8 T lymphocytes are key components of the cellular immunity against human cytomegalovirus ( HCMV ) , a widespread pathogen that cause severe illness and poor outcome in immunocom...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "blood", "cells", "urinary", "system", "procedures", "medicine", "and", "health", "sciences", "organ", "transplantation", "immune", "cells", "pathology", "and", "laboratory", "medicine", "pathogens", "immunology", "microbiology", "cytomegalovirus", "infection", "cloning",...
2018
HCMV triggers frequent and persistent UL40-specific unconventional HLA-E-restricted CD8 T-cell responses with potential autologous and allogeneic peptide recognition
HIV-1 undergoes multiple rounds of error-prone replication between transmission events , resulting in diverse viral populations within and among individuals . In addition , the virus experiences different selective pressures at multiple levels: during the course of infection , at transmission , and among individuals . ...
The speed at which HIV-1 evolves within individuals and across epidemics is substantially different . Identifying the mechanisms shaping this phenomenon has important implications for understanding disease severity and transmission of HIV-1 , especially when considering the spread of viruses that are resistant to drugs...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "organismal", "evolution", "medicine", "and", "health", "sciences", "pathology", "and", "laboratory", "medicine", "viral", "transmission", "and", "infection", "pathogens", "geographical", "locations", "microbiology", "uganda", "retroviruses", "viruses", "immunodeficiency", ...
2018
Evolution of HIV-1 within untreated individuals and at the population scale in Uganda
The intricate shaping of the facial skeleton is essential for function of the vertebrate jaw and middle ear . While much has been learned about the signaling pathways and transcription factors that control facial patterning , the downstream cellular mechanisms dictating skeletal shapes have remained unclear . Here we p...
The exquisite functions of the vertebrate face require the precise formation of its underlying bones . Remarkably , many of the genes required to shape the facial skeleton are the same from fish to man . In this study , we use the powerful zebrafish system to understand how the skeletal components of the face acquire d...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "and", "health", "sciences", "condensation", "face", "bmp", "signaling", "condensed", "matter", "physics", "vertebrates", "animals", "notch", "signaling", "animal", "models", "osteichthyes", "immune", "receptor", "signaling", "developmental", "biology", "mod...
2016
Competition between Jagged-Notch and Endothelin1 Signaling Selectively Restricts Cartilage Formation in the Zebrafish Upper Face
Sensory processing in the brain includes three key operations: multisensory integration—the task of combining cues into a single estimate of a common underlying stimulus; coordinate transformations—the change of reference frame for a stimulus ( e . g . , retinotopic to body-centered ) effected through knowledge about a...
Over the first few years of their lives , humans ( and other animals ) appear to learn how to combine signals from multiple sense modalities: when to “integrate” them into a single percept , as with visual and proprioceptive information about one's body; when not to integrate them ( e . g . , when looking somewhere els...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "circuit", "models", "neural", "networks", "computational", "neuroscience", "psychophysics", "sensory", "systems", "biology", "computational", "biology", "sensory", "perception", "neuroscience", "learning", "and", "memory", "coding", "mechanisms" ]
2013
Learning Multisensory Integration and Coordinate Transformation via Density Estimation
Blocking Plasmodium falciparum transmission to mosquitoes has been designated a strategic objective in the global agenda of malaria elimination . Transmission is ensured by gametocyte-infected erythrocytes ( GIE ) that sequester in the bone marrow and at maturation are released into peripheral blood from where they are...
Malaria transmission is ensured by deformable mature gametocyte-infected erythrocytes being taken up when a mosquito bites . Non-deformable immature gametocyte stages are sequestered in the bone marrow , as their lack of deformability would lead to their splenic clearance . In the present study , we apply nano-filtrati...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
cAMP-Signalling Regulates Gametocyte-Infected Erythrocyte Deformability Required for Malaria Parasite Transmission
Scabies is a parasitic skin infestation caused by the burrowing mite Sarcoptes scabiei . It is common worldwide and spreads rapidly under crowded conditions , such as those found in socially disadvantaged communities of Indigenous populations and in developing countries . Pruritic scabies lesions facilitate opportunist...
Scabies , a neglected parasitic disease caused by the microscopic mite Sarcoptes scabiei , is a major driving force behind bacterial skin infections in tropical settings . Aboriginal and Torres Strait Islander peoples are nearly twenty times more likely to die from acute rheumatic fever and rheumatic heart disease than...
[ "Abstract", "Introduction", "Methods", "Results", "and", "Discussion" ]
[ "infectious", "diseases/bacterial", "infections", "infectious", "diseases/neglected", "tropical", "diseases", "infectious", "diseases/tropical", "and", "travel-associated", "diseases", "infectious", "diseases/skin", "infections" ]
2010
A Tractable Experimental Model for Study of Human and Animal Scabies
Trypanosoma cruzi , causative agent of Chagas disease in humans and dogs , is a vector-borne zoonotic protozoan parasite that can cause fatal cardiac disease . While recognized as the most economically important parasitic infection in Latin America , the incidence of Chagas disease in the United States of America ( US ...
Trypanosoma cruzi is a protozoan parasite that causes Chagas disease in humans and dogs and may eventually lead to mortalities related to cardiac failure . This parasite is most frequently transmitted by triatomine bug vectors , commonly called “kissing bugs . ” Although Chagas disease is predominately acquired in Lati...
[ "Abstract", "Introduction", "Methods", "Results", "Discussion" ]
[]
2016
Molecular Diversity of Trypanosoma cruzi Detected in the Vector Triatoma protracta from California, USA
We define an interface-interaction network ( IIN ) to capture the specificity and competition between protein-protein interactions ( PPI ) . This new type of network represents interactions between individual interfaces used in functional protein binding and thereby contains the detail necessary to describe the competi...
Much of the work inside the cell is carried out by proteins interacting with other proteins . Each edge in a protein-protein interaction network reflects these functional interactions and each node a separate protein , creating a complex structure that nevertheless follows well-established global and local patterns rel...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "macromolecular", "complex", "analysis", "systems", "biology", "biochemistry", "physics", "proteomic", "databases", "protein", "interactions", "proteins", "protein", "structure", "macromolecular", "assemblies", "biology", "computational", "biology", "proteomics", "biophysics"...
2013
Interface-Resolved Network of Protein-Protein Interactions
Zinc is an essential micronutrient for all living organisms and is involved in a plethora of processes including growth and development , and immunity . However , it is unknown if there is a common genetic and molecular basis underlying multiple facets of zinc function . Here we used natural variation in Arabidopsis th...
Plants have evolved mechanisms to cope with complex environments in which resources as well as potential threats are fluctuating . Thereby , plants modulate their growth based on multiple cues from the environment . In this study , by exploring natural genetic variation in Arabidopsis to study the role of zinc in regul...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "methods" ]
[ "genome-wide", "association", "studies", "plant", "growth", "and", "development", "brassica", "plant", "physiology", "developmental", "biology", "plant", "science", "model", "organisms", "experimental", "organism", "systems", "genome", "analysis", "seedlings", "plants", ...
2018
Natural allelic variation of the AZI1 gene controls root growth under zinc-limiting condition
Streptococcal toxic shock syndrome ( STSS ) is a severe invasive infection characterized by the sudden onset of shock and multiorgan failure; it has a high mortality rate . Although a number of studies have attempted to determine the crucial factors behind the onset of STSS , the responsible genes in group A Streptococ...
Group A streptococcus ( GAS ) causes life-threatening severe invasive diseases , including necrotizing fasciitis and streptococcal toxic shock-like syndrome . Although many studies have attempted to determine factors that are crucial for the onset of streptococcal toxic shock syndrome ( STSS ) , bacterial factors respo...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "genetics", "and", "genomics/disease", "models", "genetics", "and", "genomics/genetics", "of", "disease", "microbiology/medical", "microbiology", "infectious", "diseases/bacterial", "infections" ]
2010
Highly Frequent Mutations in Negative Regulators of Multiple Virulence Genes in Group A Streptococcal Toxic Shock Syndrome Isolates
Aberrant DNA methylation disrupts normal gene expression in cancer and broadly contributes to oncogenesis . We previously developed MethylMix , a model-based algorithmic approach to identify epigenetically regulated driver genes . MethylMix identifies genes where methylation likely executes a functional role by using t...
To elucidate the molecular basis of cancer we examine the variation and dynamics characterizing the flow of information from epigenome to the transcriptome and proteome . Conducting the first genome wide analysis of epigenome-proteome associations , we present a MethylMix analysis that leverages protein abundance data ...
[ "Abstract", "Introduction", "Results", "Discussion", "Methods" ]
[ "medicine", "and", "health", "sciences", "breast", "tumors", "cancer", "risk", "factors", "cancers", "and", "neoplasms", "protein", "abundance", "oncology", "epigenetics", "dna", "medical", "risk", "factors", "dna", "methylation", "chromatin", "research", "and", "an...
2019
The impact of DNA methylation on the cancer proteome
The exosome complex functions in RNA metabolism and transcriptional gene silencing . Here , we report that mutations of two Arabidopsis genes encoding nuclear exosome components AtRRP6L1 and AtRRP6L2 , cause de-repression of the main flowering repressor FLOWERING LOCUS C ( FLC ) and thus delay flowering in early-flower...
Arabidopsis FLOWERING LOCUS C ( FLC ) delays flowering; therefore , repressing expression of FLC provides a critical mechanism to regulate flowering . This mechanism involves multiple levels of regulation , including genetic regulation by transcription factors , and epigenetic regulation by modifications of genomic DNA...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "biology", "and", "life", "sciences" ]
2014
Arabidopsis RRP6L1 and RRP6L2 Function in FLOWERING LOCUS C Silencing via Regulation of Antisense RNA Synthesis
The effectiveness of a mass vaccination program can engender its own undoing if individuals choose to not get vaccinated believing that they are already protected by herd immunity . This would appear to be the optimal decision for an individual , based on a strategic appraisal of her costs and benefits , even though sh...
A major factor underlying the success of voluntary vaccination schemes is the public perception about the costs and benefits associated with vaccines . Individuals may avoid vaccination if they perceive the risk of infection to be low compared to the potential hazards and inconveniences associated with getting vaccinat...
[ "Abstract", "Introduction", "Model", "Results", "Discussion" ]
[ "medicine", "and", "health", "sciences", "infectious", "disease", "epidemiology", "applied", "mathematics", "immunology", "sociology", "social", "sciences", "vaccines", "preventive", "medicine", "social", "epidemiology", "probability", "distribution", "mathematics", "networ...
2019
Epidemic prevalence information on social networks can mediate emergent collective outcomes in voluntary vaccine schemes
Clinical strains of HCMV encode 20 putative ORFs within a region of the genome termed ULb′ that are postulated to encode functions related to persistence or immune evasion . We have previously identified ULb′-encoded pUL138 as necessary , but not sufficient , for HCMV latency in CD34+ hematopoietic progenitor cells ( H...
Human cytomegalovirus is a ubiquitous herpesvirus that , like all herpesviruses , establishes a life long relationship with its host through a latent infection . The molecular basis of viral latency is poorly understood , in part , because viral determinants of latency and the corresponding virus-host interactions are ...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "medicine", "biochemistry", "infectious", "diseases", "biology", "microbiology", "molecular", "cell", "biology" ]
2011
A Novel Human Cytomegalovirus Locus Modulates Cell Type-Specific Outcomes of Infection
Appropriate diagnostics to monitor disease trends and assess the effectiveness and impact of interventions are essential for guiding treatment strategies at different thresholds of schistosomiasis transmission and for certifying elimination . Field validation of these assays is urgently needed before they can be adopte...
Immunodiagnostic assays are widely applied in the field to control schistosomiasis in P . R . China as the prevalence and infection intensity of schistosome infections decrease . Field evaluations are urgently needed before they can be adopted to support policy decisions of the national programme for the control and el...
[ "Abstract", "Introduction", "Materials", "and", "Methods", "Results", "Discussion" ]
[ "medicine", "infectious", "diseases", "test", "evaluation", "schistosomiasis", "research", "monitoring", "diagnostic", "medicine", "science", "policy", "neglected", "tropical", "diseases", "government", "laboratories", "parasitic", "diseases", "research", "assessment", "res...
2011
Tools to Support Policy Decisions Related to Treatment Strategies and Surveillance of Schistosomiasis Japonica towards Elimination
Fe-S bound proteins are ubiquitous and contribute to most basic cellular processes . A defect in the ISC components catalyzing Fe-S cluster biogenesis leads to drastic phenotypes in both eukaryotes and prokaryotes . In this context , the Frataxin protein ( FXN ) stands out as an exception . In eukaryotes , a defect in ...
Iron sulfur ( Fe-S ) clusters are ubiquitous cofactors found in proteins which function in very diverse pathways ranging from respiration to DNA repair . The mitochondrial Fe-S biogenesis machinery ISC was inherited from the bacterial ancestor of mitochondria . In both prokaryotes and eukaryotes , deficiency of core IS...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[]
2015
Turning Escherichia coli into a Frataxin-Dependent Organism
Organisms evolve two routes to surviving infections—they can resist pathogen growth ( resistance ) and they can endure the pathogenesis of infection ( tolerance ) . The sum of these two properties together defines the defensive capabilities of the host . Typically , studies of animal defenses focus on either understand...
To boost its defenses , an organism may increase its resistance to infection by reducing the fitness of the invading pathogen; alternatively , the host may increase its tolerance by reducing the damage caused by a given quantity of pathogen . Melanization is an immune response that has been linked to defense in the fly...
[ "Abstract", "Introduction", "Results", "Discussion", "Materials", "and", "Methods" ]
[ "infectious", "diseases", "immunology" ]
2008
A Signaling Protease Required for Melanization in Drosophila Affects Resistance and Tolerance of Infections